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ap 1 transcription factors c jun  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ap 1 transcription factors c jun
    Ap 1 Transcription Factors C Jun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ap 1 transcription factors c jun/product/Cell Signaling Technology Inc
    Average 96 stars, based on 693 article reviews
    ap 1 transcription factors c jun - by Bioz Stars, 2026-03
    96/100 stars

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    Image Search Results


    Representation of melatonin’s effects on UVB-induced skin photoaging. UV, ultraviolet; EGFR: epidermal growth factor receptor; ROS: reactive oxygen species; MDA, malondialdehyde; GSSG/GSH, oxidized/reduced glutathione; JNK, c-Jun amino-terminal kinase; AP-1, activator protein-1; MMP-1, matrix metalloproteinase-1; MMP-3, matrix metalloproteinase-3; TIMP-1, tissue inhibitor of metalloproteinase-1; PIPC, type I procollagen.

    Journal: Life

    Article Title: Melatonin Prevents UVB-Induced Skin Photoaging by Inhibiting Oxidative Damage and MMP Expression through JNK/AP-1 Signaling Pathway in Human Dermal Fibroblasts

    doi: 10.3390/life12070950

    Figure Lengend Snippet: Representation of melatonin’s effects on UVB-induced skin photoaging. UV, ultraviolet; EGFR: epidermal growth factor receptor; ROS: reactive oxygen species; MDA, malondialdehyde; GSSG/GSH, oxidized/reduced glutathione; JNK, c-Jun amino-terminal kinase; AP-1, activator protein-1; MMP-1, matrix metalloproteinase-1; MMP-3, matrix metalloproteinase-3; TIMP-1, tissue inhibitor of metalloproteinase-1; PIPC, type I procollagen.

    Article Snippet: The transcriptional activity of AP-1 (c-Jun/c-Fos) was detected by highly sensitive ELISA-based TransAM AP-1 c-Fos/c-Jun transcription factor assay kits (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Techniques:

    UBA6 is required for the inhibition of NF-κB activation in CD8 + T cells. Sorted naïve CD8 + T cells were activated with soluble anti-CD3 and anti-CD28 Ab for the indicated times. ( A , B ) Cells were activated in the presence of 50 μM cycloheximide. Whole cell lysates were prepared and analyzed by western blot analysis using p-IκBα, IκBα, and UBA6 Abs to detect these proteins after ( A ) shot time and ( B ) long time stimulation with anti-CD3 and anti-CD28 Ab. ( C ) Flow cytometry analyses of phospho-p65 the levels in CD4 and CD8 T cells. Cells were unstimulated (left) or stimulated for 15 min (right) (left panel). MFI of phosphor-p65 levels are shown (right panel) ( n = 4, two-way ANOVA, mean ± SEM, ** p < 0.01). ( D ) Nuclear transcription factor NF-κB p65 activity was measured by ELISA-based TransAM NF-κB p65 kit (Active Motif) according to the manufacturer’s protocol. Data are representative of three independent experiments.

    Journal: Cells

    Article Title: Ubiquitin Activating Enzyme UBA6 Regulates Th1 and Tc1 Cell Differentiation

    doi: 10.3390/cells11010105

    Figure Lengend Snippet: UBA6 is required for the inhibition of NF-κB activation in CD8 + T cells. Sorted naïve CD8 + T cells were activated with soluble anti-CD3 and anti-CD28 Ab for the indicated times. ( A , B ) Cells were activated in the presence of 50 μM cycloheximide. Whole cell lysates were prepared and analyzed by western blot analysis using p-IκBα, IκBα, and UBA6 Abs to detect these proteins after ( A ) shot time and ( B ) long time stimulation with anti-CD3 and anti-CD28 Ab. ( C ) Flow cytometry analyses of phospho-p65 the levels in CD4 and CD8 T cells. Cells were unstimulated (left) or stimulated for 15 min (right) (left panel). MFI of phosphor-p65 levels are shown (right panel) ( n = 4, two-way ANOVA, mean ± SEM, ** p < 0.01). ( D ) Nuclear transcription factor NF-κB p65 activity was measured by ELISA-based TransAM NF-κB p65 kit (Active Motif) according to the manufacturer’s protocol. Data are representative of three independent experiments.

    Article Snippet: To detect transcription factors (phospho-p65 and phospho-c-Jun), we followed the manufacturer’s protocol from eBioscience (San Diego, CA, USA).

    Techniques: Inhibition, Activation Assay, Western Blot, Flow Cytometry, Activity Assay, Enzyme-linked Immunosorbent Assay